Publications

2019
Harris RM, Kao H-Y, Alarcon JM, Hofmann HA, Fenton AA. Hippocampal transcriptomic responses to enzyme-mediated cellular dissociation. Hippocampus [Internet]. 2019 :1-7. Publisher's VersionAbstract

Single-neuron gene expression studies may be especially important for understanding nervous system structure and function because of the neuron-specific functionality and plasticity that defines functional neural circuits. Cellular dissociation is a prerequi- site technical manipulation for single-cell and single cell-population studies, but the extent to which the cellular dissociation process affects neural gene expression has not been determined. This information is necessary for interpreting the results of experi- mental manipulations that affect neural function such as learning and memory. The goal of this research was to determine the impact of cellular dissociation on brain transcriptomes. We compared gene expression of microdissected samples from the dentate gyrus (DG), CA3, and CA1 subfields of the mouse hippocampus either prepared by a standard tissue homogenization protocol or subjected to enzymatic digestion used to dissociate cells within tissues. We report that compared to homoge- nization, enzymatic dissociation alters about 350 genes or 2% of the hippocampal transcriptome. While only a few genes canonically implicated in long-term potentiation and fear memory change expression levels in response to the dissociation procedure, these data indicate that sample preparation can affect gene expression profiles, which might confound interpretation of results depending on the research question. This study is important for the investigation of any complex tissues as research effort moves from subfield level analysis to single cell analysis of gene expression.

harris_et_al-2019-hippocampus.pdf
Friesen CN, Hofmann HA. Molecular origins and outcomes of status and stress in primates. Proceedings of the National Academy of Sciences USA [Internet]. 2019;116 (4) :1081-1083. Publisher's Version friesenhofmann_2019.pdf
Solomon-Lane TK, Hofmann HA. Early-life social environment alters juvenile behavior and neuroendocrine function in a highly social cichlid fish. bioRxiv preprint [Internet]. 2019. Publisher's VersionAbstract
Early-life experiences can shape adult behavior, with consequences for fitness and health, yet fundamental questions remain unanswered about how early-life social experiences are translated into variation in brain and behavior. The African cichlid fish Astatotilapia burtoni, a model system in social neuroscience, is well known for its highly plastic social phenotypes in adulthood. Here, we rear juveniles in either social groups or pairs to investigate the effects of early-life social environments on behavior and neuroendocrine gene expression. We find that both juvenile behavior and neuroendocrine function are sensitive to early-life effects. Behavior robustly co-varies across multiple contexts (open field, social cue investigation, and dominance behavior assays) to form a behavioral syndrome, with pair-reared juveniles towards the end of syndrome that is less active and socially interactive. Pair-reared juveniles also submit more readily as subordinates. In a separate cohort, we measured whole brain expression of stress and sex hormone genes. Expression of glucocorticoid receptor (GR) 1a was elevated in group-reared juveniles, supporting a highly-conserved role for the stress axis mediating early-life effects. The effect of rearing environment on androgen receptor (AR) and estrogen receptor (ER) expression was mediated by treatment duration (1 vs. 5 weeks). Finally, expression of corticotropin-releasing factor (CRF) and GR2 decreased significantly over time. Rearing environment also caused striking differences in gene co-expression, such that expression was tightly integrated in pair-reared juveniles, but not group-reared or isolates. Together, this research demonstrates the important developmental origins of behavioral phenotypes and identifies potential behavioral and neuroendocrine mechanisms.
solomon-lane_hofmann.pdf
Young RL, Ferkin MH, Ockendon NF, Orr VN, Phelps SM, Pogány Á, Richards-Zawacki CL, Summers K, Székely T, Trainor BC, et al. Conserved transcriptomic profiles underpin monogamy across vertebrates. Proc. Natl. Acad. Sci. USA [Internet]. 2019;116 (4) :1331-1336. Publisher's VersionAbstract
Social monogamy, typically characterized by the formation of a pair bond, increased territorial defense, and often biparental care, has evolved numerous times in animals. Despite the independent evolutionary origins of monogamous mating systems, several homologous brain regions and neuroendocrine pathways play conserved roles in regulating social affiliation and parental care, but little is known about the evolution of the neuromolecular mechanisms underlying monogamy. Here, we show that shared transcriptomic profiles are associated with monogamy across vertebrates and discuss the importance of our discovery for understanding the origins of behavioral diversity. We compare neural transcriptomes of reproductive males in monogamous and nonmonogamous species pairs of mice, voles, parid songbirds, frogs, and cichlid fishes. Our results provide evidence of a universal transcriptomic code underlying monogamy in vertebrates.
young_etal_2019_pnas.pdf youngetal2019_pnas_supportinginfo.pdf
Nugent BM, Stiver KA, Hofmann HA, Alonzo SH. Experimentally‐induced variation in neuroendocrine processes affects male reproductive behavior, sperm characteristics, and social interactions. Molecular Ecology [Internet]. 2019;In Press. Publisher's VersionAbstract

While extensive research has focused on how social interactions evolve, the fitness consequences of the neuroendocrine mechanisms underlying these interactions have rarely been documented, especially in the wild. Here, we measure how the neuroendocrine mechanisms underlying male behavior affecting mating success and sperm competition in the ocellated wrasse (Symphodus ocellatus). In this species, males exhibit three alternative reproductive types. ‘Nesting males’ provide parental care, defend territories, and form cooperative associations with unrelated ‘satellites’, who cheat by sneaking fertilizations but help by reducing sperm competition from ‘sneakers’ who do not cooperate or provide care. To measure the fitness consequences of the mechanisms underlying these social interactions, we used “phenotypic engineering” that involved administering an androgen receptor antagonist (flutamide) to wild, free-living fish. Nesting males treated with flutamide shifted their aggression from sneakers to satellite males and experienced decreased submissiveness by sneaker males (which correlated with decreased nesting male mating success). The preoptic area (POA), a region controlling male reproductive behaviors, exhibited dramatic down-regulation of androgen receptor (AR) and vasotocin 1a receptor (V1aR) mRNA following experimental manipulation of androgen signaling. We did not find a direct effect of the manipulation on male mating success, paternity or larval production. However, variation in neuroendocrine mechanisms generated by the experimental manipulation was significantly correlated with changes in behavior and mating success: V1aR expression was negatively correlated with satellite-directed aggression and expression of its ligand arginine vasotocin (AVT) was positively correlated with courtship and mating success, thus revealing the potential for sexual selection on these mechanisms.

nugent_et_al-2018-molecular_ecology.pdf
2018
Kabelik D, Hofmann HA. Comparative neuroendocrinology: A call for more study of reptiles!. Hormones and Behavior [Internet]. 2018;106 :189-192. Publisher's Version kabelikhofmann_2018.pdf
Goldsby HJ, Young RL, Hofmann HA, Hintze A. Serendipitous Scaffolding to improve a Genetic Algorithm's Speed and Quality, in Genetic and Evolutionary Computation Conference (GECCO). Kyoto (Japan) ; 2018 :959-966. Publisher's VersionAbstract
A central challenge to evolutionary computation is enabling techniques to evolve increasingly complex target end products. Frequently direct approaches that reward only the target end product itself are not successful because the path between the starting conditions and the target end product traverses through a complex fitness landscape, where the directly accessible intermediary states may be require deleterious or even simply neutral mutations. As such, a host of techniques have sprung up to support evolutionary computation techniques taking these paths. One technique is scaffolding where intermediary targets are used to provide a path from the starting state to the end state. While scaffolding can be successful within well-understood domains it also poses the challenge of identifying useful intermediaries. Within this paper we first identify some shortcomings of scaffolding approaches --- namely, that poorly selected intermediaries may in fact hurt the evolutionary computation's chance of producing the desired target end product. We then describe a light-weight approach to selecting intermediate scaffolding states that improve the efficacy of the evolutionary computation.
goldsby_et_al_2018.pdf
Li CY, Hofmann HA, Harris ML, Earley RL. Real or fake? Natural and artificial social stimuli elicit divergent behavioral and neural responses in the mangrove rivulus fish, Kryptolebias marmoratus. Proc. R. Soc. B. [Internet]. 2018;285 (20181610). Publisher's VersionAbstract
Understanding how the brain processes social information and generates adaptive behavioural responses is a major goal in neuroscience. We examined behaviour and neural activity patterns in socially relevant brain nuclei of hermaphroditic mangrove rivulus fish (Kryptolebias marmoratus) provided with different types of social stimuli: stationary model opponent, regular mirror, non-reversing mirror and live opponent. We found that: (i) individuals faced with a regular mirror were less willing to interact with, delivered fewer attacks towards and switched their orientation relative to the opponent more frequently than fish exposed to a non-reversing mirror image or live opponent; (ii) fighting with a regular mirror image caused higher expression of immediate-early genes (IEGs: egr-1 and c-Fos) in the teleost homologues of the basolateral amygdala and hippocampus, but lower IEG expression in the preoptic area, than fighting with a non-reversing mirror image or live opponent; (iii) stationary models elicited the least behavioural and IEG responses among the four stimuli; and (iv) the nonreversing mirror image and live opponent drove similar behavioural and neurobiological responses. These results suggest that the various stimuli provide different types of information related to conspecific recognition in the context of aggressive contests, which ultimately drive different neurobiological responses.
li_et_al_2018.pdf
Kabelik D, Weitekamp CA, Choudhury SC, Hartline JT, Smith AN, Hofmann HA. Neural activity in the social decision-making network of the brown anole during reproductive and agonistic encounters. Hormones and Behavior [Internet]. 2018;106 :178-188. Publisher's VersionAbstract
Animals have evolved flexible strategies that allow them to evaluate and respond to their social environment by integrating the salience of external stimuli with internal physiological cues into adaptive behavioral responses. A highly conserved social decision-makingnetwork (SDMN), consisting of interconnected social behavior and mesolimbic reward networks, has been proposed to underlie such adaptive behaviors across all vertebrates, although our understanding of this system in reptiles is very limited. Here we measure neural activation across the SDMN and associated regions in the male brown anole (Anolis sagrei), within both reproductive and agonistic contexts, by quantifying the expression density of the immediate early gene product Fos. We then relate this neural activity measure to social context, behavioral expression, and activation (as measured by colocalization with Fos) of different phenotypes of ‘source’ node neurons that produce neurotransmittersand neuropeptides known to modulate SDMN ‘target’ node activity. Our results demonstrate that measures of neural activation across the SDMN network are generally independent of specific behavioral output, although Fos induction in a few select nodes of the social behavior network component of the SDMN does vary with social environment and behavioral output. Under control conditions, the mesolimbic reward nodes of the SDMN actually correlate little with the social behavior nodes, but the interconnectivity of these SDMN components increases dramatically within a reproductive context. When relating behavioral output to specific source node activation profiles, we found that catecholaminergic activation is associated with the frequency and intensity of reproductive behavior output, as well as with aggression intensity. Finally, in terms of the effects of source node activation on SDMN activity, we found that Ile8-oxytocin (mesotocin) populations correlate positively, while Ile3-vasopressin (vasotocin), catecholamine, and serotonin populations correlate negatively with SDMN activity. Taken together, our findings present evidence for a highly dynamic SDMN in reptiles that is responsive to salient cues in a social context-dependent manner.
kabelik_et_al_2018.pdf
Solomon-Lane TK, Hofmann HA. Early-life social environment alters juvenile behavior and neuroendocrine function in a highly social cichlid fish. 2018.Abstract
Early-life experiences can shape adult behavior, with consequences for fitness and health, yet fundamental questions remain unanswered about how early social environments and experiences are translated into variation in brain and behavior. The African cichlid fish Astatotilapia burtoni, a model system in social neuroscience, is well known for its highly plastic social phenotypes in adulthood. Here, we rear juveniles in either social groups or pairs to investigate the effects of early-life social environments on behavior and neuroendocrine gene expression. We find that both juvenile behavior and neuroendocrine function are sensitive to early-life social effects. Behavior robustly co-varies across multiple contexts (open field, social cue investigation, and dominance behavior assays) to form a behavioral syndrome. Rearing environment shifts pair-reared juveniles towards the end of syndrome that is less active and socially interactive. Pair-reared juveniles also submit more readily as subordinates. In a separate cohort, we then measured neural expression for stress and sex hormone genes, signaling systems known to be developmentally plastic and involved in translating environmental conditions into biological responses and regulating adult social behavior. Rearing environment causes striking differences in neuroendocrine gene co-expression networks. Specifically, expression was tightly integrated in pair-reared juveniles, but not group-reared or isolated juveniles. Glucocorticoid receptor subtypes 1a, 1b, and 2, as well as androgen receptor , drive the significant differences between treatment groups, which supports a highly conserved role for the stress axis mediating early-life effects. Together, this research demonstrates the important developmental origins of behavioral phenotypes and identifies potential behavioral and neuroendocrine mechanisms.
solomon-lane_and_hofmann_2018.pdf
Renn SCP, Machado HE, Duftner N, Sessa AK, Harris RM, Hofmann HA. Gene expression signatures of mating system evolution. Genome [Internet]. 2018. Publisher's VersionAbstract

The diversity of mating systems among animals is astounding. Importantly, similar mating systems have evolved even across distantly related taxa. However, our understanding of the mechanisms underlying these convergently evolved phenotypes is limited. Here, we examine on a genomic scale the neuromolecular basis of social organization in Ectodini cichlids from Lake Tanganyika. Using field collected males and females of four closely related species representing two independent evolutionary transitions from polygyny to monogamy, we take a comparative transcriptomic approach to test the hypothesis that these independent transitions have recruited similar gene sets. Our results demonstrate that while lineage and species exert a strong influence on neural gene expression profiles, social phenotype can also drive gene expression evolution. Specifically, 331 genes (~6% of those assayed) were associated with monogamous mating systems independent of species or sex. Among these genes, we find a strong bias (4:1 ratio) toward genes with increased expression in monogamous individuals. A highly conserved nonapeptide system known to be involved in the regulation of social behavior across animals was not associated with mating system in our analysis. Overall, our findings suggest deep molecular homologies underlying the convergent or parallel evolution of monogamy in different lineages of Ectodini cichlids.

renn_et_al_2018.pdf
Wylie DC, Hofmann HA, BV Z. SArKS: Discovering gene expression regulatory motifs and domains by suffix array kernel smoothing. PLOS Computational Biology [Internet]. 2018. Publisher's VersionAbstract

 Experiments designed to assess differential gene expression represent a rich resource for discovering how DNA regulatory sequences influence transcription. Results derived from such experiments are usually quantified as continuous scores, such as fold changes, test statistics and p -values. We present a de novo motif discovery algorithm, SArKS, which uses a nonparametric kernel smoothing approach to identify promoter motifs correlated with elevated differential expression scores. SArKS has the capability to smooth over both motif sequence similarity and, in a second pass, over spatial proximity of multiple motifs to identify longer regions enriched in correlative motifs. We applied SArKS to simulated data, illustrating how SArKS can be used to find motifs embedded in random background sequences, and to two published RNA-seq expression data sets, one probing S. cerevisiae  transcriptional response to anti-fungal agents and the other comparing gene expression profiles among cortical neuron subtypes in M. musculus. For both RNA-seq sets we successfully identified motifs whose kernel-smoothed scores were significantly elevated compared to the permutation-estimated background distributions. We found strong similarities between these identified motifs and known, biologically meaningful sequence elements which may help to provide additional context for the results previously published regarding these data sets. Finally, because eukaryotic transcription regulation is highly combinatorial, we also outline how SArKS methods might be extended to discover synergistic motifs

wylie_et_al._2018.pdf
2017
Goldsby HJ, Young RL, Hofmann HA, Hintze A. Increasing the complexity of solutions produced by an evolutionary developmental system., in Genetic and Evolutionary Computation Conference (GECCO). Berlin (Germany) ; 2017 :57-58.Abstract

Evolutionary computation and neuroevolution seek to create systems of ever increasing sophistication, such that the digitally evolved forms reflect the variety, diversity, and complexity seen within nature in living organisms. In general, most evolutionary computation and neuroevolution techniques do so by encoding the final form without any type of development. This is in contrast to nature, where most complex organisms go through a developmental period. Here we focus on an evolving digital tissues that develop from a single cell and unfold into a complex body plan. It quickly became evident that evolving developing forms is quite challenging. We compare four different techniques that have successfully been employed within evolutionary computation to evolve complex forms and behavior: scaffolding (i.e., gradually increasing the difficulty of the task rewarded by the environment over evolutionary time), stepping stones (i.e., rewarding easier tasks within an environment that can co-opted for the performance of more complex tasks), and island models (i.e., rewarding different fitness functions within different subpopulations with migration). We show the effect of these methods on the evolution of complex forms that develop from a single cell, the rate of adaptation, and different dimensions of robustness and variation among solutions.

goldsby_et_al_2017.pdf
Baker MR, Hofmann HA, Wong RY. Neurogenomics of behavioural plasticity in socioecological contexts. In: Encyclopedia of Life Sciences. Chichester: John Wiley & Sons, Ltd ; 2017. Publisher's VersionAbstract

Social and ecological challenges often elicit behavioural and physiological responses that are adaptive and subject to selection. The varying behavioural states and traits of animals are a direct output of the nervous system and underlying molecular substrates. Changes in gene expression in response to a variety of contexts such as mate choice, aggression and developmental experience can alter a number of cellular and neural pathways that lead to changes in behaviour. A common framework has emerged to understand the role of the transcriptome in animal behaviour. Behavioural plasticity describes both an individual’s ability to modify behavioural states and correlated suites of behaviour in populations, which may constrain variance across contexts. By integrating the study of behavioural plasticity with genome scale, bioinformatics and candidate gene analyses, we are rapidly expanding our understanding of this kind of organismal flexibility, its relationship with the genome and its evolutionary implications.

baker_etal_els_2017.pdf
Rodriguez-Santiago M, Nguyen J, Winton LS, Weitekamp CA, Hofmann HA. Arginine vasotocin preprohormone is expressed in surprising regions of the teleost forebrain. Frontiers in Endocrinology [Internet]. 2017;8. Publisher's VersionAbstract
Nonapeptides play a fundamental role in the regulation of social behavior, among numerous other functions. In particular, arginine vasopressin and its non-mammalian homolog, arginine vasotocin (AVT), have been implicated in regulating affiliative, reproductive, and aggressive behavior in many vertebrate species. Where these nonapeptides are synthesized in the brain has been studied extensively in most vertebrate lineages. While several hypothalamic and forebrain populations of vasopressinergic neurons have been described in amniotes, the consensus suggests that the expression of AVT in the brain of teleost fish is limited to the hypothalamus, specifically the preoptic area (POA) and the anterior tuberal nucleus (putative homolog of the mammalian ventromedial hypothalamus). However, as most studies in teleosts have focused on the POA, there may be an ascertainment bias. Here, we revisit the distribution of AVT preprohormone mRNA across the dorsal and ventral telencephalon of a highly social African cichlid fish. We first use in situ hybridization to map the distribution of AVT preprohormone mRNA across the telencephalon. We then use quantitative real-time polymerase chain reaction to assay AVT expression in the dorsomedial telencephalon, the putative homolog of the mammalian basolateral amygdala. We find evidence for AVT preprohormone mRNA in regions previously not associated with the expression of this nonapeptide, including the putative homologs of the mammalian extended amygdala, hippocampus, striatum, and septum. In addition, AVT preprohormone mRNA expression within the basolateral amygdala homolog differs across social contexts, suggesting a possible role in behavioral regulation. We conclude that the surprising presence of AVT preprohormone mRNA within dorsal and medial telencephalic regions warrants a closer examination of possible AVT synthesis locations in teleost fish, and that these may be more similar to what is observed in mammals and birds.
rodriguez-santiago_et_al._2017.pdf
Weitekamp CA, Nguyen J, Hofmann HA. Neuromolecular Regulation of Aggression Differs by Social Role during Joint Territory Defense. Integrative and Comparative Biology. 2017 :1-9.Abstract

In response to a territory intrusion, neighboring males of the African cichlid fish Astatotilapia burtoni engage in aggressive joint territory defense in a manner that depends on their social role. Here, we examine the possible function of several neuroendocrine and neuromodulator pathways previously implicated in the regulation of complex social behavior. We find that the neuromolecular regulation of aggression during joint territory defense is very much dependent on an individual’s role in this context. In neighbors but not in residents, aggression is correlated to gene expression in the medial part of the dorsal telencephalon (area Dm), the putative homolog to the mammalian basolateral amygdala. This correlation is strikingly high for expression of the serotonin receptor 5-HT2c, suggesting the serotonin system is important in regulating context-dependent behavior. Furthermore, by examining candidate gene expression co-variance patterns in area Dm and in the lateral part of the dorsal telencephalon (area Dl), the putative homolog to the mammalian hippocampus, we identify two main patterns: gene expression is co-regulated within, but not across, brain regions, and co-regulation is synergistic rather than antagonistic. Our results highlight the critical effect of social context on both behavior and its neuromolecular basis.

weitekamp_et_al._2017_icb.pdf
Weitekamp CA, Nguyen J, Hofmann HA. Social context affects behavior, preoptic area gene expression, and response to D2 receptor manipulationduring territorial defense in a cichlid fish. Genes, Brain and Behavior. 2017;16 :601-611.Abstract

Social context often has profound effects on behavior, yet the neural and molecular mechanisms which mediate flexible behavioral responses to different social environments are not well understood. We used the African cichlid fish, Astatotilapia burtoni, to examine aggressive defense behavior across three social contexts representing different motivational states: a reproductive opportunity, a familiar male and a neutral context.To elucidate how differences in behavior across con-texts may be mediated by neural gene expression, we examined gene expression in the preoptic area, a brain region known to control male aggressive and sexual behavior. We show that social context has broad effects on preoptic gene expression. Specifically, we found that the expression of genes encoding nonapeptides and sex steroid receptors are upregulated in the familiar male context. Furthermore, circulating levels of testosterone and cortisol varied markedly depending on social context. We also manipulated the D2 receptor (D2R) ineach social context, given that it has been implicated in mediating context-dependent behavior. We found that a D2R agonist reduced intruder-directed aggression in the reproductive opportunity and familiar male contexts, while a D2R antagonist inhibited intruder-directed aggression in the reproductive opportunity context and increased aggression in the neutral context. Our results demonstrate a critical role for preoptic gene expression, as well as circulating steroid hormone levels, in encoding information from the social environment and in shaping adaptive behavior. In addition, they provide further evidence for a role of D2R in context-dependent behavior.

weitekamp_et_al-2017.pdf
Liebeskind BJ, Hofmann HA, Hillis DA, Zakon HH. Evolution of Animal Neural Systems. Annual Review of Ecology, Evolution, and Systematics. 2017;48 :377-398.Abstract

Nervous systems are among the most spectacular products of evolution. Their provenance and evolution have been of interest and often the subjects of intense debate since the late nineteenth century. The genomics era has provided researchers with a new set of tools with which to study the early evolution of neurons, and recent progress on the molecular evolution of the first neurons has been both exciting and frustrating. It has become increasingly obvious that genomic data are often insufficient to reconstruct complex phenotypes in deep evolutionary time because too little is known about how gene function evolves over deep time. Therefore, additional functional data across the animal tree are a prerequisite to a fuller understanding of cell evolution. To this end, we review the functional modules of neurons and the evolution of their molecular components, and we introduce the idea of hierarchical molecular evolution.

liebeskind_et_al_2017.pdf
Dijkstra PD, Maguire SM, Harris RM, Rodriguez AA, DeAngelis RS, Flores SA, Hofmann HA. The melanocortin system regulates body pigmentation and social behaviour in the cichlid fish Astatotilapia burtoni. Proc R Soc B. 2017. dijkstra_et_al._2017.pdf
Weitekamp CA, Solomon-Lane TK, Del Valle P, Triki Z, Nugent BM, Hofmann HA. A role for oxytocin-like receptor in social habituation in a teleost. Brain, Behavior, and Evolution. 2017;89 :153-161.Abstract

Oxytocin (OT) mediates social habituation in rodent model systems, but its role in mediating this effect in other vertebrates is unknown. We used males of the African cichlid fish, Astatotilapia burtoni , to investigate two aspects of isotocin (IT; an OT homolog) signaling in social habituation. First, we examined the expression of IT receptor 2 (ITR2) as well as two immediate early genes in brain regions implicated in social recognition. Next, we examined IT neuron activity using immunohistochemistry. Patterns of gene expression in homologs of the amygdala and hippocampus implicate IT signaling in these regions in social habituation to a territorial neighbor. In the preoptic area, the expression of the ITR2 subtype and IT neuron activity respond to the presence of a male, independent of familiarity. Our results implicate IT in mediating social habituation in a teleost.

weitekamp_et_al_2017.pdf

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